Journal: Nutrients

Article Title: Vitamin C Activates Osteoblastogenesis and Inhibits Osteoclastogenesis via Wnt/β-Catenin/ATF4 Signaling Pathways

doi: 10.3390/nu11030506

Figure Lengend Snippet: Expression of both osteoblast- and osteoclast-regulated proteins in vitamin C-treated rat tibias. ( A ) Western blot image of Wnt3a, β-catenin, and ATF4 and quantitative assay of Wnt3a, β-catenin, and ATF4 protein expression in vitamin C-treated rat tibias. ( B ) Western blot image of p-AKT, p-ERK, p-p38, and p-JNK and quantitative assay of p-AKT, p-ERK, p-p38, and p-JNK protein expression in vitamin C-treated rat tibias. Expression was quantified using ImageJ software relative to that of β-actin. Values represent the mean ± standard deviation. Values with different letters were significantly different according to Duncan’s multiple range test ( P < 0.05).

Article Snippet: Membranes were blocked with 5% bovine serum albumin prior to incubation with specific primary antibodies against BMP-2, RUNX2, Wnt3a, osteocalcin, COL-1 (Abcam, Cambridge, UK), SMAD1/5/8 (Santa Cruz Biotechnology, Dallas, TX, USA), ATF4 (Boster, Pleasanton, CA, USA), osteoprotegerin (OPG), RANK, RANKL (Bioss Antibodies, Woburn, MA, USA), TRAP, cathepsin K (GeneTex, Irvine, CA, USA), β-catenin, phosphorylated serine/threonine kinase (p-AKT), phosphorylated extracellular signal-regulated kinase (p-ERK), p-p38, phosphorylated c-Jun N-terminal kinase (p-JNK), and β-actin (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Western Blot, Software, Standard Deviation

Journal: Oncology reports

Article Title: Gonadotropins promote human ovarian cancer cell migration and invasion via a cyclooxygenase 2-dependent pathway.

doi: 10.3892/or.2017.5784

Figure Lengend Snippet: Figure 1. FSHR and LHR were expressed in SKOV3 and HO8910 cells but did not affect cell proliferation and apoptosis. Expression of FSHR and LHR was examined by immunocytochemical staining (A), RT-PCR (B), and western blotting (C). HO8910 and SKOV3 cells were treated with FSH and LH (0, 100, and 500 mIU/ml) alone or in combination, but no changes in proliferation (D and E) and apoptosis (F) were observed.

Article Snippet: The cells were then incubated with FSHR antibody (cat. PB1120; 1:200, Boster) or LHR antibody (cat. A01120; 1:500, Boster) at 4 ̊C overnight.

Techniques: Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot